目的

观察模拟户外光照的全光谱动态光源对豚鼠透镜诱导性近视眼屈光发育的影响。

方法

选取1周龄，体质量≤100 g英国种三色豚鼠57只，采用数字表法随机将其分为持续照明组、低频变化组及高频变化组。采用全光谱Solux光源模拟户外光照，并通过电子调控设备实现光照强度以余弦方式动态波动。持续照明组接受光照强度为8000 lux照射，低频变化组接受光照强度为500～8000 lux，变化频率为10 min/0.05 Hz；高频变化组接受光照强度为500～8000 lux，变化频率为20 s/0.002 Hz。所有豚鼠均双眼配戴－6.00 D透镜，分别于实验前后3周测量豚鼠眼的屈光度、前房深度、晶状体厚度、玻璃体腔长度及眼轴长度等眼球生物参数变化。屈光度、前房深度、晶状体厚度、玻璃体深度及眼轴长度均符合正态分布，采用¯x±s描述，组间比较采用方差分析。实验前后眼部参数的变化采用配对t检验，不同光照模式的眼部参数实验前后的变化采用t检验。

结果

实验前，持续照明组、低频变化组及高频变化豚鼠眼屈光度分别为（3.55±0.53）D、（3.66±0.58）D及（3.49±0.49）D，组间比较差异无统计学意义（F＝0.69，P＞0.05）。实验前，持续照明组豚鼠前房深度、晶状体厚度、玻璃体腔长度及眼轴长度分别为（1.09±0.05）mm、（2.46±0.14）mm、（3.40±0.18）mm及（7.23±0.09）mm；低频变化组分别为（1.08±0.05）mm、（2.47±0.15）mm、（3.41±0.19）mm及（7.18±0.08）mm；高频变化组分别为（1.11±0.04）mm、（2.49±0.15）mm、（3.44±0.16）mm及（7.23±0.09）mm。组间比较的差异无统计学意义（F＝1.22，0.36，0.19，2.10；P＞0.05）。实验3周后，低频变化组豚鼠眼屈光度较实验前增加（－2.43±0.68）D，高频照明组增加（－1.34±0.91）D，持续照明组增加（－1.62±0.95）D。其中，高频变化组较实验前增加最少，持续照明组和低频变化组次之。与低频变化组相比，高频变化组豚鼠眼屈光度呈相对性远视状态，两组豚鼠屈光度变化的比较差异有统计学意义（t＝－4.35，P＜0.05）。各组间豚鼠眼晶状体厚度、眼轴长度、前房深度及玻璃体腔长度实验前后变化的比较差异无统计学意义（F＝0.32，2.10，1.05，1.10；P＞0.05）。

结论

高频变化的动态Solux光照能有效地减缓豚鼠透镜诱导性近视眼的进展。但与持续照明相比，高频动态变化近视眼保护作用无强度依赖性。

Objective

The aim of this study is to observe the effect of dynamic full spectrum light onmyopia progression of lens-induced guinea pigs.

Methods

Fifty seven one week old British trichrome guinea pigs weighing≤100 g were randomly divided into continuous illumination group（18 guinea pigs），low-frequency change group（20 guinea pigs）and high-frequency change group（19 guinea pigs）.The full spectrum SoLux light source was used to simulate outdoor lighting，and the light intensity was dynamically fluctuated in a cosine manner through electronic control equipment.The continuous illumination group received light intensity of 8000 lux，and the low-frequency change group received light intensity of 500 to 8000 lux，with a change frequency of 10 min/0.05 Hz.The high-frequency change group was exposed to 500 to8000 luxwith a frequency of20 s/0.002 Hz.All guinea pigswere equipped with－6.00 D lenses in both eyes.The changes of ocular biological parameters such as diopter，anterior chamber depth，lens thickness，vitreous cavity length，and axial length were measured before and 3 weeks after the experiment.Diopter，anterior chamber depth，lens thickness，vitreous depth and axial length were all in linewith normal distribution，and described by（¯x±s），and performed with analysis of variance between groups.The changes of eye parameters before and after the experimentwere detected by paired t-test，and the changes of eye parameters between groups with different light modes or dynamic light sources with different change frequencies were compared by t test.

Results

Before the experiment，refractive error of the constant lighting group was（3.55±0.53）D.The low frequency dynamic light group was（3.66±0.58）D and the high frequency dynamic light group was（3.49±0.49）D.There was no difference in refractive error among all groups.After3 weeks treatment，all the groups of guinea pigs developedmyopia.The low frequency dynamic light group developed tomyopia by（－2.43±0.68）D，followed by constant lighting group（－1.62±0.95）D.Before the experiment，the visual acuity of guinea pigs in the continuous illumination group，lowfrequency variation group，and high-frequency variation group were（3.55±0.53）D，（3.66±0.58）D，and（3.49±0.49）D，respectively.There was no statistically significant difference between the groups（F＝0.69，P＞0.05）.Before the experiment，the anterior chamber depth，lens thickness，vitreous cavity length，and axial length of the guinea pigs in the continuous lighting group were（1.09±0.05）mm，（2.46±0.14）mm，（3.40±0.18）mm，and（7.23±0.09）mm，respectively.The low-frequency variation groups were（1.08±0.05）mm，（2.47±0.15）mm，（3.41±0.19）mm，and（7.18±0.08）mm，respectively.The high-frequency change groups were（1.11±0.04）mm，（2.49±0.15）mm，（3.44±0.16）mm，and（7.23±0.09）mm，respectively.There was no statistically significant difference in the biological parameters of guinea pig eyeballs between groups（F＝1.22，0.36，0.19，2.10；P＞0.05）.After 3 weeks of experimental treatment，the refractive power of guinea pigs in the low-frequency change group increased by（－2.43±0.68）D compared to before the experiment，the highfrequency illumination group increased by（－1.34±0.91）D，and the continuous illumination group increased by（－1.62±0.95）D.The high-frequency variation group showed the least increase compared to before the experiment，followed by the continuous illumination group and the low-frequency variation group.Compared with the low-frequency change group，the high-frequency change group of guinea pigs showed a relative hyperopia state in terms of refractive power，and the difference in refractive power changes between the two groups of guinea pigs was statistically significant（t＝－4.35，P＜0.05）.There was no statistically significant difference in the changes of lens thickness，axial length，anterior chamber depth，and vitreous cavity length among the groups of guinea pigs（F＝0.32，2.10，1.05，1.10；P＞0.05）.

Conclusions

High frequency dynamic illumination can effectively slow down the progression of lens-induced myopia in guinea pigs.However，compared with constant lighting，high frequency dynamic lighting did not show greater protective effect againstmyopia.The results of this experiment need further research and exploration.