The relationship between cytokine level in aqueous humor and diabetes mellitus with cataract and age-related cataract

doi:10.3877/cma.j.issn.2095-2007.2019.05.008

Abstract

Abstract:

Objective

This study was aim to investigate the changes of factors in aqueous humor of diabetes mellitus with cataract, and to analyze their relationship with cataract of diabetic patients.

Methods

Clinical data of 49 patients (49 eyes) receiving cataract surgery in the affiliated hospital of Inner Mongolia Medical University from October 2017 to February 2018 were collected, including 14 patients (14 eyes) with diabetes mellitus with cataract as the experimental group and 35 patients (35 eyes) with simple senile cataract as the cataract group. Among of the experimental group, the age was 54 to 75, the average age was (64.8±10.7). Among of the cataract group, the age was 55 to 72, the average age was (64.0±8.8). All the patients conduct preoperative collection of fasting venous blood and intraoperative collection of aqueous humor. Using cytometric bead array to detect vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), interleukin-8 (IL-8), interleukin-10 (IL-10) and vascular cell adhesion molecule-1 (VCAM-1) and using enzyme-linked immuno-sorbent assay to detect vascular endothelial growth factor B (VEGF-B) and placental growth factor (PLGF). Spearman′s rank correlation coefficient was used to detect the correlation aqueous humour levels of IL-8, IL-10, VEGF, bFGF, PLGF, VCAM-1 and VEGF-B with cortical opacity, nuclear opacity and posterior subcapsular opacity in both groups. Spearman′s rank correlation coefficient was also used to detect the correlation serum levels of IL-8, IL-10, VEGF, bFGF, VCAM-1 and VEGF-B with cortical opacity, nuclear opacity and posterior subcapsular opacity between two groups.

Results

The aqueous humor concentrations of IL-8, IL-10, VEGF, BFGF, VCAM-1, PLGF and VEGF-B in the experimental 1 and 2 group were (31.69±27)pg/ml, (5.2±0.41) pg/ml, (47.27±20.58) pg/ml, (17.28±4.11) pg/ml, (416.5±310.66) pg/ml, (17.85±6.70) pg/ml, (65.54±26.89) pg/ml, (12.38±6.52) pg/ml, (3.7±2.13) pg/ml, (36.54±14.97) pg/ml, (17.43±4.33) pg/ml, (331.39±205.80) pg/ml, (22.21±17.47) pg/ml, (74.36±26.75) pg/ml, respectively. The concentration of VCAM-1 in aqueous humor was negatively correlated with nuclear opacity in the experimental 1 group (r=-0.61, P<0.05). The concentration of all factors in serum was not correlated with nuclear opacity in the experimental 2 group (r=-0.03, -0.10, 0.26, 0.04, -0.32, -0.19; P>0.05), (r=-0.15, 0.13, -0.15, -0.11, 0.11, -0.18; P>0.05), (r=0.21, 0.53, 0.20, 0.13, -0.12, 0.16; P>0.05), (r=-0.06, 0.03, -0.01, -0.20, 0.09, 0.20; P>0.05), (r=0.39, 0.02, -0.11, -0.20, -0.13, 0.03; P>0.05) and (r=0.13, 0.09, 0.32, 0.25, 0.11, -0.10; P>0.05).

Conclusion

The concentration of VCAM-1 in aqueous humor of diabetic patients was negatively correlated with the nuclear opacity, and VCAM-1 may act as a protective factor for nuclear cataract.

Key words: Vascular cell adhesion molecules-1, Cataract, Diabetes mellitus with cataract

Xiaojuan Wang, Fei Hong, Dayong Yang, Dan Zhu, Yong Tao. The relationship between cytokine level in aqueous humor and diabetes mellitus with cataract and age-related cataract[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2019, 09(05): 312-319.

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References 67

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组别	眼数	白细胞介素-8	白细胞介素-10	血管内皮生长因子	重组人碱性成纤维细胞生长因子	血管细胞粘附分子-1	胎盘生长因子	人血管内皮细胞生长因子B
实验1组	14	31.69±0.41	5.2±0.41	47.27±20.58	17.28±4.11	416.50±310.66	17.85±6.70	65.54±26.89
实验2组	35	12.38±6.52	3.7±2.13	36.54±14.97	17.43±4.33	331.39±205.80	22.21±17.47	74.36±26.75
t/z		-3.75^b	-1.63^b	-2.03^a	-0.20^b	-0.95^a	-0.40^b	-1.11^b
P值		<0.05	>0.05	<0.05	>0.05	>0.05	>0.05	>0.05

组别	眼数	白细胞介素-8	白细胞介素-10	血管内皮生长因子	重组人碱性成纤维细胞生长因子	血管细胞粘附分子-1	胎盘生长因子	人血管内皮细胞生长因子B
实验1组	14	31.69±0.41	5.2±0.41	47.27±20.58	17.28±4.11	416.50±310.66	17.85±6.70	65.54±26.89
实验2组	35	12.38±6.52	3.7±2.13	36.54±14.97	17.43±4.33	331.39±205.80	22.21±17.47	74.36±26.75
t/z		-3.75^b	-1.63^b	-2.03^a	-0.20^b	-0.95^a	-0.40^b	-1.11^b
P值		<0.05	>0.05	<0.05	>0.05	>0.05	>0.05	>0.05

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