The construction of a mouse dry eye model and its corneal feature detection

doi:10.3877/cma.j.issn.2095-2007.2023.01.003

Abstract

Abstract:

Objective

To explore the construction of a mouse dry eye model and its corneal feature detection method.

Methods

A total of 12 healthy pathogen free (SPF) grade C57BL/6 mice, aged 6 to 8 weeks, male, weighing (20±1) g were selected. Using the number table method, 12 mice were randomly divided into an experimental group and a control group, with 6 mice in each group. The right eye of each mouse was selected for the experiment. Six mice (6 eyes) in the experimental group were given a 0.2% benzalkonium chloride solution 5 μL in their right eyes to construct a mouse dry eye model with local eye drops, twice a day at 9: 00 and 21: 00, lasting for 7 days; 6 mice in the control group (6 eyes) were given phosphate buffer solution 5 μ L in the right eye local eye drops were used as a normal control, with eye drops taken twice daily at 9: 00 and 21: 00, lasting for 7 days. At 7 days, corneal fluorescein staining was performed on the right eye of mice in the experimental and control groups using an animal ophthalmic slit lamp microscope, and corneal fluorescein staining scores were performed. The ISOCT ophthalmic ultra microscopic imaging system was used to perform optical coherence tomography (AS-OCT) examination of the anterior segment of the right eye of experimental and control group mice, and the thickness of the central corneal epithelial layer was measured. The corneal fluorescence staining score and the thickness of the central corneal epithelial layer conform to the normal distribution, and were described by (±s), and compared by the unpaired t test.

Results

Seven days after modeling with 0.2% benzalkonium chloride solution, the results of corneal fluorescein staining imaging showed that the transparency of the right eye cornea of the experimental group mice decreased and spot staining, while the cornea of the normal control group was transparent, without obvious abnormal changes. The fluorescein staining score of the right eye cornea of the experimental group mice increased compared with the control group, which were (10.833±0.98) points and (1.833±0.98) points, respectively, with a statistically significant difference (t=15.85, P<0.05). AS-OCT examination results showed that the right corneal epithelium of mice in the experimental group was deformed, with thickness defects and high reflection punctate corneal epithelial infiltration. The thickness of the central corneal epithelial layer of mice in the experimental group was significantly lower than that in the control group, which were (33.00±6.64) μ m and (44.45±4.34) μm, respectively. The difference was statistically significant (t=3.53, P<0.05).

Conclusions

The mouse dry eye model induced by 0.2% benzalkonium chloride solution is relatively stable, and AS-OCT is suitable for detecting the characteristics of the benzalkonium chloride induced mouse dry eye model. Its main features are corneal epithelial deformation, thickness defects, high reflex point like epithelial infiltration, and significant thinning of the central corneal epithelial layer.

Key words: Mice, Dry eye disease, Anterior segment optical coherence tomography, Imaging characteristics

Jingyi Bai, Xuan Huang, Yiquan Zhang, Ying Tian, Yong Tao. The construction of a mouse dry eye model and its corneal feature detection[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2023, 13(01): 12-17.

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References 30

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