Advances in the application of blue light induced-oxidative damage models in the study of ophthalmic diseases

doi:10.3877/cma.j.issn.2095-2007.2022.03.008

Abstract

Abstract:

Blue light with the wavelength at 400 nm to 500 nm could cause some damages to different tissues from the eye surface to the fundus because of its high energy, thus causing a series of ophthalmic diseases related to oxidative stress. At present, animal experiments in vivo and cell models in vitro using blue light irradiation to explore the damage of eye tissue had been reported. The domestic and foreign literature published in recent years about the damage caused by blue light wavelengths, the irradiation intensity and irradiation time were reviewed, and the molecular mechanism of damage were analyzed, which to improve the cognition, advise eye protection, and promote to develop drugs for the oxidative damage from blue light to the eyes.

Key words: Blue light cell model, Blue light animal model, Ophthalmic diseases, Oxidative damage, Molecular mechanisms

Jinyu Jiang, Donglei Zhang, Wei He. Advances in the application of blue light induced-oxidative damage models in the study of ophthalmic diseases[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2022, 12(03): 168-172.

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References 52

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蓝光波长(nm)	实验对象	实验条件	模型	结论	参考文献
400~420	C57BL/6小鼠	50 J/cm²，连续照射10 d	干眼	小鼠泪膜破裂时间降低，眼表组织炎性因子白细胞介素－1β及白细胞介素－6表达增多	[16]
	人角膜上皮细胞	>10 J/cm²	干眼	含有五味子、白芷及边沁等提取物能调节细胞内抗氧化物酶活性，降低过度产生的活性氧	[19]
421~440	Balb/c小鼠	10 000 lux/d，1 h/d，连续14 d，	年龄相关性黄斑变性	小鼠视网膜发生氧化损伤，且夏枯草提取物能够提高其抗氧化物酶活性，降低脂质过氧化物水平	[45]
	人视网膜色素上皮细胞	添加脂褐素光敏剂		脂褐素光敏剂N－亚视黄基－N－视黄基－乙醇胺可加速蓝光对视网膜色素上皮细胞的损伤	[46,51]
441~460	SD大鼠	照射12周	白内障	SD大鼠晶状体凋亡标志物半胱氨酸蛋白酶－1、半胱氨酸蛋白酶－11及焦亡蛋白表达增加	[25]
	人工晶状体上皮细胞	2500 lux，5 h		人工晶状体上皮细胞凋亡	[25]
	视网膜神经前体细胞R28	1000 lux，12 h		细胞线粒体裂解蛋白和融合蛋白表达异常，细胞线粒体功能受损	[33]
	人视网膜色素上皮细胞	(4±0.5)mW/cm²，5~6 h		细胞损伤是细胞凋亡或坏死的节点；脂褐素光敏剂可增加细胞内的凋亡蛋白、紧密连接蛋白－1以及血管内皮生长因子的表达	[41,50]
461~480	SD大鼠	500 lux/d，12 h/d，连续5 d	白内障	SD大鼠晶状体内抗氧化酶活性下降	[24]
	小鼠光感受器细胞	450 lux，6 h；添加越橘提取物和花青素		细胞S－视蛋白表达升高，添加越橘提取物和花青素后抗氧化作用明显	[30]
	人视网膜色素上皮细胞	(2000±500) lux，12 h；添加枸杞多糖		细胞氧化损伤，添加枸杞多糖后能够增强色素上皮细胞线粒体膜电位，有抗氧化作用	[42]
	Balb/c小鼠	10 000 lux，1 h/d，连续14 d	年龄相关性黄斑变性	小鼠视网膜厚度降低，视网膜内核转录因子－κB和脱氧核糖核酸修复酶的裂解增加	[46,47]

蓝光波长(nm)	实验对象	实验条件	模型	结论	参考文献
400~420	C57BL/6小鼠	50 J/cm²，连续照射10 d	干眼	小鼠泪膜破裂时间降低，眼表组织炎性因子白细胞介素－1β及白细胞介素－6表达增多	[16]
	人角膜上皮细胞	>10 J/cm²	干眼	含有五味子、白芷及边沁等提取物能调节细胞内抗氧化物酶活性，降低过度产生的活性氧	[19]
421~440	Balb/c小鼠	10 000 lux/d，1 h/d，连续14 d，	年龄相关性黄斑变性	小鼠视网膜发生氧化损伤，且夏枯草提取物能够提高其抗氧化物酶活性，降低脂质过氧化物水平	[45]
	人视网膜色素上皮细胞	添加脂褐素光敏剂		脂褐素光敏剂N－亚视黄基－N－视黄基－乙醇胺可加速蓝光对视网膜色素上皮细胞的损伤	[46,51]
441~460	SD大鼠	照射12周	白内障	SD大鼠晶状体凋亡标志物半胱氨酸蛋白酶－1、半胱氨酸蛋白酶－11及焦亡蛋白表达增加	[25]
	人工晶状体上皮细胞	2500 lux，5 h		人工晶状体上皮细胞凋亡	[25]
	视网膜神经前体细胞R28	1000 lux，12 h		细胞线粒体裂解蛋白和融合蛋白表达异常，细胞线粒体功能受损	[33]
	人视网膜色素上皮细胞	(4±0.5)mW/cm²，5~6 h		细胞损伤是细胞凋亡或坏死的节点；脂褐素光敏剂可增加细胞内的凋亡蛋白、紧密连接蛋白－1以及血管内皮生长因子的表达	[41,50]
461~480	SD大鼠	500 lux/d，12 h/d，连续5 d	白内障	SD大鼠晶状体内抗氧化酶活性下降	[24]
	小鼠光感受器细胞	450 lux，6 h；添加越橘提取物和花青素		细胞S－视蛋白表达升高，添加越橘提取物和花青素后抗氧化作用明显	[30]
	人视网膜色素上皮细胞	(2000±500) lux，12 h；添加枸杞多糖		细胞氧化损伤，添加枸杞多糖后能够增强色素上皮细胞线粒体膜电位，有抗氧化作用	[42]
	Balb/c小鼠	10 000 lux，1 h/d，连续14 d	年龄相关性黄斑变性	小鼠视网膜厚度降低，视网膜内核转录因子－κB和脱氧核糖核酸修复酶的裂解增加	[46,47]

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