Effects of a two-enzyme inducer on apoptosis in rat Müller cells in the high glucose environment

doi:10.3877/cma.j.issn.2095-2007.2019.06.005

Abstract

Abstract:

Objective

This aim of this study was to investigate the effect of 5, 6-dihydrocyclopentene-1, 2-dithiol-3-thione, a two-enzyme inducer(CPDT)on the apoptosis of rat Müller cells in the high glucose environment and whether it exerts its role through the apoptosis-related genes Bcl-2/Bax pathways, in order to explore the prospect of CPDT in the prevention and treatment of diabetic retinopathy.

Methods

SD rat Müller cells were cultured in vitro and randomly divided into 3 groups: control group (25 mM DMEM), high glucose group (65 mM DMEM), CPDT intervention group (65 mM DMEM + 60 uM CPDT). These cells were cultured for 72 h. Apoptosis and cell cycle changes were detected by the flow cytometry with double staining. The expressions of Bcl-2 and Bax proteins in each group were detected by Western blotting. Data were collected and one-way ANOVA was used to performstatistically analyzed between groups.

Results

The results of double staining flow cytometry showed that the apoptotic rate of high glucose group was (17.6±3.16%), that of control group was (8.25±0.26%), and that of CPDT intervention group was (13.5%±2.25%). There was statistically significant among them (F=13.48, P<0.05). The results of Western blotting hybridization detection showed that compared with the control group, the expression of Bcl-2 protein in the high glucose group was significantly decreased and the expression of Bax protein was significantly increased (t=150.38, 234.46; P<0.05). Compared with the high glucose group, the expression of Bcl-2 protein in the CPDT intervention group increased, Bax protein expression decreased (t=108.03, 36.33; P<0.05).

Conclusion

High glucose could up-regulate Bax, down-regulate Bcl-2 expression, decrease Bcl-2/Bax ratio, and promote apoptosis. CPDT could increase the activity of rat Müller cells, Bcl-2 expression and Bcl-2/Bax ratio, inhibit the expression of Bax from the pressure of high glucose, thereby inhibiting the apoptosis of rat Müller cells induced by high glucose, and had a protective effect on Müller cells in a high glucose environment.

Key words: Diabetic retinopathy, Müller cells, CPDT, Bcl-2/Bax, Apoptosis

Xiaoqiang Wu, Wei He, Qi Huang, Hongbin Lyn. Effects of a two-enzyme inducer on apoptosis in rat Müller cells in the high glucose environment[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2019, 09(06): 348-353.

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References 40

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