Application of reflection confocal microscopy in diagnosis and treatment of eyelid tumor

doi:10.3877/cma.j.issn.2095-2007.2018.03.007

Abstract

Abstract:

Reflection confocal microscope(RCM) is a new high-resolution imaging microscope for noninvasive observation of living tissues. It is also a new non-invasive imaging examination method. This method can observe the skin tissue hierarchy and lesion characteristics. The surgical treatment of eyelid tumor involves the beauty of the face and the retention of the function of the eyelids, so it is very important for the preliminary identification of benign and malignant tumors, the nature of the tumor and the preliminary determination of the boundary before the eyelid operation. Different eyelid masses have their own characteristics under the RCM, so the RCM plays a certain role in the preliminary screening of eyelid mass, and can be applied to the diagnosis of eyelid mass and the follow-up of postoperative palpebral mass, and it has a good prospect in clinical application.

Key words: Reflection confocal microscope, Eyelid tumor, Basal cell carcinoma, Malignant melanoma, Squamous cell carcinoma

Hang Song, Yan Zhang, Xiaodan Jiang, Xiaoyan Zhang, Xuemin Li. Application of reflection confocal microscopy in diagnosis and treatment of eyelid tumor[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2018, 08(03): 134-139.

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References 65

[1]	Diaconeasa A, Boda D, Neagu M, et al. The role of confocal microscopy in the dermato－oncology practice[J]. J Med Life, 2011, 4(1): 63-74.
[2]	Rajadhyaksha M, Gonzalez S, Zavislan JM, et al. In vivo confocal scanning laser microscopy of human skin Ⅱ：advances in instrumentation and comparison with histology[J]. J Invest Dermatol, 1999, 113(3): 293-303.
[3]	Que SK, Fraga－Braghiroli N, Grant－Kels JM, et al. Through the looking glass: Basics and principles of reflectance confocal microscopy[J]. J Am Acad Dermatol, 2015, 73(2): 276-284.
[4]	Rajadhyaksha M, Grossman M, Esterowitz D, et al. In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast[J]. J Invest Dermatol, 1995, 104(6): 946-952.
[5]	Chen CS, Sierra H, Cordova M, et al. Confocal microscopy－guided laser ablation for superficial and early nodular Basal cell carcinoma: a promising surgical alternative for superficial skin cancers[J]. JAMA Dermatol, 2014, 150(9): 994-998.
[6]	Hibler BP, Cordova M, Wong RJ, et al. Intraoperative real－time reflectance confocal microscopy for guiding surgical margins of lentigo maligna melanoma[J]. Dermatol Surg, 2015, 41(8): 980-983.
[7]	Ulrich M, Krueger－Corcoran D, Roewert－Huber J, et al. Reflectance confocal microscopy for noninvasive monitoring of therapy and detection of subclinical actinic keratoses[J]. Dermatology, 2010, 220(1): 15-24.
[8]	Torres A, Niemeyer A, Berkes B, et al. 5% imiquimod cream and reflectance－mode confocal microscopy as adjunct modalities to Mohs micrographic surgery for treatment of basal cell carcinoma[J]. Dermatol Surg, 2004, 30(12): 1462-1469.
[9]	Nadiminti H, Scope A, Marghoob AA, et al. Use of reflectance confocal microscopy to monitor response of lentigo maligna to nonsurgical treatment[J]. Dermatol Surg, 2010, 36(2): 177-184.
[10]	Ahlgrimm－Siess V, Horn M, Koller S, et al. Monitoring efficacy of cryotherapy for superficial basal cell carcinomas with in vivo reflectance confocal microscopy: a preliminary study[J]. J Dermatol Sci, 2009, 53(1): 60-64.
[11]	Pe'Er J. Pathology of eyelid tumors[J]. Indian J Ophthalmol, 2016, 64(3): 177-190.
[12]	Guitera P, Menzies SW, Longo C, et al. In vivo confocal microscopy for diagnosis of melanoma and basal cell carcinoma using a two－step method: analysis of 710 consecutive clinically equivocal cases[J]. J Invest Dermatol, 2012, 132(10): 2386-2394.
[13]	Webber SA, Wurm EM, Douglas NC, et al. Effectiveness and limitations of reflectance confocal microscopy in detecting persistence of basal cell carcinomas: a preliminary study[J]. Australas J Dermatol, 2011, 52(3): 179-185.
[14]	Peppelman M, Wolberink EA, Blokx WA, et al. In vivo diagnosis of basal cell carcinoma subtype by reflectance confocal microscopy[J]. Dermatology, 2013, 227(3): 255-262.
[15]	Chuah SY, Tee SI, Tan WP, et al. Reflectance confocal microscopy is a useful non－invasive tool in the in vivo diagnosis of pigmented basal cell carcinoma in Asians[J]. Australas J Dermatol, 2017, 58(2): 130-134.
[16]	Gonzalez S, Tannous Z. Real－time, in vivo confocal reflectance microscopy of basal cell carcinoma[J]. J Am Acad Dermatol, 2002, 47(6): 869-874.
[17]	Terui Y, Mishima Y, Sugimura N, et al. Identification of CD20 C－terminal deletion mutations associated with loss of CD20 expression in non－Hodgkin's lymphoma[J]. Clinical Cancer Research, 2009, 15(7): 2523-2530.
[18]	Pellacani G, Pepe P, Casari A, et al. Reflectance confocal microscopy as a second－level examination in skin oncology improves diagnostic accuracy and saves unnecessary excisions: a longitudinal prospective study[J]. Br J Dermatol, 2014, 171(5): 1044-1051.
[19]	Ahlgrimm－Siess V, Cao T, Oliviero M, et al. The vasculature of nonmelanocytic skin tumors in reflectance confocal microscopy: vascular features of basal cell carcinoma[J]. Arch Dermatol, 2010, 146(3): 353-354.
[20]	Gareau DS, Karen JK, Dusza SW, et al. Sensitivity and specificity for detecting basal cell carcinomas in Mohs excisions with confocal fluorescence mosaicing microscopy[J]. J Biomed Opt, 2009, 14(3): 34012.
[21]	Bennassar A, Vilata A, Puig S, et al. Ex vivo fluorescence confocal microscopy for fast evaluation of tumour margins during Mohs surgery[J]. Br J Dermatol, 2014, 170(2): 360-365.
[22]	Kadouch DJ, Schram ME, Leeflang MM, et al. In vivo confocal microscopy of basal cell carcinoma: a systematic review of diagnostic accuracy[J]. J Eur Acad Dermatol Venereol, 2015, 29(10): 1890-1897.
[23]	Cinotti E, Perrot JL, Campolmi N, et al. The role of in vivo confocal microscopy in the diagnosis of eyelid margin tumors: 47 cases[J]. J Am Acad Dermatol, 2014, 71(5): 912-918.
[24]	Longo C, Farnetani F, Ciardo S, et al. Is confocal microscopy a valuable tool in diagnosing nodular lesions? A study of 140 cases[J]. Br J Dermatol, 2013, 169(1): 58-67.
[25]	Propperova I, Langley RG. Reflectance－mode confocal microscopy for the diagnosis of sebaceous hyperplasia in vivo[J]. Arch Dermatol, 2007, 143(1): 134.
[26]	Rajadhyaksha M, Marghoob A, Rossi A, et al. Reflectance confocal microscopy of skin in vivo: From bench to bedside[J]. Lasers Surg Med, 2017, 49(1): 7-19.
[27]	Star P, Guitera P. Lentigo Maligna, Macules of the Face, and Lesions on Sun－Damaged Skin: Confocal Makes the Difference[J]. Dermatol Clin, 2016, 34(4): 421-429.
[28]	Cinotti E, Haouas M, Grivet D, et al. In vivo and Ex vivo Confocal Microscopy for the Management of a Melanoma of the Eyelid Margin[J]. Dermatol Surg, 2015, 41(12): 1437-1440.
[29]	Losi A, Longo C, Cesinaro AM, et al. Hyporeflective pagetoid cells: a new clue for amelanotic melanoma diagnosis by reflectance confocal microscopy[J]. Br J Dermatol, 2014, 171(1): 48-54.
[30]	Guitera P, Menzies SW, Argenziano G, et al. Dermoscopy and in vivo confocal microscopy are complementary techniques for diagnosis of difficult amelanotic and light－coloured skin lesions[J]. Br J Dermatol, 2016, 175(6): 1311-1319.
[31]	Guitera P, Pellacani G, Longo C, et al. In vivo reflectance confocal microscopy enhances secondary evaluation of melanocytic lesions[J]. J Invest Dermatol, 2009, 129(1): 131-138.
[32]	Langley RG, Walsh N, Sutherland AE, et al. The diagnostic accuracy of in vivo confocal scanning laser microscopy compared to dermoscopy of benign and malignant melanocytic lesions: a prospective study[J]. Dermatology, 2007, 215(4): 365-372.
[33]	Busam KJ, Hester K, Charles C, et al. Detection of clinically amelanotic malignant melanoma and assessment of its margins by in vivo confocal scanning laser microscopy[J]. Arch Dermatol, 2001, 137(7): 923-929.
[34]	Stevenson AD, Mickan S, Mallett S, et al. Systematic review of diagnostic accuracy of reflectance confocal microscopy for melanoma diagnosis in patients with clinically equivocal skin lesions[J]. Dermatol Pract Concept, 2013, 3(4): 19-27.
[35]	Scope A, Benvenutoandrade C, Agero AL, et al. In vivo reflectance confocal microscopy imaging of melanocytic skin lesions: consensus terminology glossary and illustrative images[J]. J Am Acad Dermatol, 2007, 57(4): 644-658.
[36]	Pellacani G, Scope A, Farnetani F, et al. Towards an in vivo morphologic classification of melanocytic nevi[J]. J Eur Acad Dermatol Venereol, 2014, 28(7): 864-872.
[37]	Segura S, Puig S, Carrera C, et al. Development of a two－step method for the diagnosis of melanoma by reflectance confocal microscopy[J]. J Am Acad Dermatol, 2009, 61(2): 216-229.
[38]	Rishpon A, Kim N, Scope A, et al. Reflectance confocal microscopy criteria for squamous cell carcinomas and actinic keratoses[J]. Arch Dermatol, 2009, 145(7): 766-772.
[39]	Nori S, Rius－Díaz F, Cuevas J, et al. Sensitivity and specificity of reflectance－mode confocal microscopy for in vivo diagnosis of basal cell carcinoma: a multicenter study[J]. Journal of the American Academy of Dermatology, 2005, 51(6):923-930.
[40]	Kim HS, Park JM, Mun JH, et al. Usefulness of Dermatoscopy for the Preoperative Assessment of the Histopathologic Aggressiveness of Basal Cell Carcinoma[J]. Annals of Dermatology, 2015, 27(6):682-687.
[41]	Alarcon I, Carrera C, Malvehy J, et al. Abstracts from the Joint Meeting of the International Society for Digital Imaging of the Skin (ISDIS), the International Confocal Group (ICG), and the International Dermoscopy Society (IDS) in conjunction with the 2014 American Academy of Dermatology, Marc[J]. Dermatology Practical & Conceptual, 2014, 4(3): 1-6.
[42]	Shin MK, Kim MJ, Baek JH, et al. Analysis of the temporal change in biophysical parameters after fractional laser treatments using reflectance confocal microscopy[J]. Skin Research & Technology, 2013, 19(1): e515.
[43]	Banzhaf CA, Wind BS, Mogensen M, et al. Spatiotemporal closure of fractional laser－ablated channels imaged by optical coherence tomography and reflectance confocal microscopy[J]. Lasers Surg Med, 2016, 48(2): 157-165.
[44]	Yang J, Wang S, Dong L, et al. Skin healing and collagen changes of rats after fractional erbium：yttrium aluminum garnet laser: observation by reflectance confocal microscopy with confirmed histological evidence[J]. Lasers in Medical Science, 2016, 31(6): 1-10.
[45]	Richters RJ, Hoogedoorn L, Uzunbajakava NE, et al. Clinical, biophysical, immunohistochemical, and in vivo reflectance confocal microscopy evaluation of the response of subjects with sensitive skin to home－use fractional non－ablative photothermolysis device[J]. Lasers in Surgery & Medicine, 2016, 48(5): 474-482.
[46]	Tsilika K, Bahadoran P, Passeron T. Superficial lymphangioma treated with fractional ablative laser: a case report with clinical and reflectance confocal microscopy evaluation[J]. Dermatologic Surgery, 2013, 39(1): 141-143.
[47]	Hibler BP, Sierra H, Cordova M, et al. Carbon dioxide laser ablation of basal cell carcinoma with visual guidance by reflectance confocal microscopy: a proof－of－principle pilot study[J]. Br J Dermatol, 2016, 174(6): 1359-1364.
[48]	Cameli N, Mariano M, Serio M, et al. Preliminary comparison of fractional laser with fractional laser plus radiofrequency for the treatment of acne scars and photoaging[J]. Dermatologic Surgery, 2014, 40(5): 553-561.
[49]	Yue X, Wang H, Li Q, et al. Application of reflectance confocal microscopy to evaluate skin damage after irradiation with an yttrium－scandium－gallium－garnet (YSGG) laser[J]. Lasers in Medical Science, 2017, 32(2): 1-8.
[50]	Banzhaf CA, Lin LL, Dang N, et al. The fractional laser－induced coagulation zone characterized over time by laser scanning confocal microscopy－A proof of concept study[J]. Lasers Surg Med, 2018, 50(1): 70-77.
[51]	Casari A, Farnetani F, De PB, et al. In vivo assessment of cytological changes by means of reflectance confocal microscopy－demonstration of the effect of topical vitamin E on skin irritation caused by sodium lauryl sulfate[J]. Contact Dermatitis, 2017, 76(3): 131-137.
[52]	Linares－Cruz G, Millot G, De CP, et al. Combined analysis of in situ hybridization, cell cycle and structural markers using reflectance and immunofluorescence confocal microscopy[J]. Histochemical Journal, 1995, 27(1): 15-23.
[53]	Clode PL, Marshall AT. Calcium localisation by X－ray microanalysis and fluorescence microscopy in larvae of zooxanthellate and azooxanthellate corals[J]. Tissue & Cell, 2004, 36(6): 379-390.
[54]	Halbhuber KJ, Scheven C, Jirikowski G, et al. Reflectance enzyme histochemistry (REH): visualization of cerium－based and DAB primary reaction products of phosphatases and oxidases in cryostat sections by confocal laser scanning microscopy[J]. Histochemistry & Cell Biology, 1996, 105(3): 239-249.
[55]	Zakharov P, Talary MS, Kolm I, et al. Full－field optical coherence tomography for the rapid estimation of epidermal thickness: study of patients with diabetes mellitus type 1[J]. Physiological Measurement, 2009, 31(2): 193-205.
[56]	Shin MK, Park JM, Lim HK, et al. Characterization of microthermal zones induced by fractional radiofrequency using reflectance confocal microscopy: a preliminary study[J]. Lasers in Surgery & Medicine, 2013, 45(8): 503-508.
[57]	Ardigo M, Cameli N, Berardesca E, et al. Characterization and evaluation of pigment distribution and response to therapy in melasma using in vivo, reflectance confocal microscopy: a preliminary study[J]. J Eur Acad Dermatol, 2010, 24(11): 1296-1303.
[58]	Trelles MA, Martínez－Carpio PA. Attenuation of acne scars using high power fractional ablative unipolar radiofrequency and ultrasound for transepidermal delivery of bioactive compounds through microchannels[J]. Lasers in Surgery & Medicine, 2014, 46(2): 152-159.
[59]	Chung VQ, Dwyer PJ, Nehal KS, et al. Use of ex vivo confocal scanning laser microscopy during Mohs surgery for nonmelanoma skin cancers[J]. Dermatol Surg, 2004, 30(12): 1470-1478.
[60]	Rajadhyaksha M, Menaker G, Flotte T, et al. Confocal Examination of Nonmelanoma Cancers in Thick Skin Excisions to Potentially Guide Mohs Micrographic Surgery Without Frozen Histopathology[J]. J Invest Dermatol, 2001, 117(5): 1137-1143.
[61]	Tannous Z, Torres A, González S. In vivo real－time confocal reflectance microscopy: a noninvasive guide for Mohs micrographic surgery facilitated by aluminum chloride, an excellent contrast enhancer[J]. Dermatologic Surgery, 2010, 29(8): 839-846.
[62]	Chen CS, Elias MK, Rajadhyaksha M, et al. Multimodal in vivo optical imaging, including confocal microscopy, facilitates presurgical margin mapping for clinically complex lentigo maligna melanoma[J]. Digest of World Core Medical Journals, 2010, 153(5): 1031-1036.
[63]	Stockfleth E, Kerl H. Guidelines for the management of actinic keratoses－Developed by the Guideline Subcommittee of the European Dermatology Forum[J]. Eur J Dermatol, 2006, 16(6): 599-606.
[64]	Busam KJ, Charles C, Lee G, et al. Morphologic Features of Melanocytes, Pigmented Keratinocytes, and Melanophages by In vivo Confocal Scanning Laser Microscopy[J]. Modern Pathology, 2001, 14(9): 862-868.
[65]	Weyers W, Bonczkowitz M, Weyers I, et al. Melanoma in situ versus melanocytic hyperplasia in sun－damaged skin. Assessment of the significance of histopathologic criteria for differential diagnosis[J]. Am J Dermatopathol, 1996, 18(6): 560-566.

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