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中华眼科医学杂志(电子版) ›› 2023, Vol. 13 ›› Issue (04) : 215 -220. doi: 10.3877/cma.j.issn.2095-2007.2023.04.005

论著

干燥综合征血清微量元素变化及其与疾病严重程度的相关性研究
郝然, 魏姗珊, 吴倩如, 李学民, 翟长斌†()   
  1. 100730　首都医科大学附属北京同仁医院　北京同仁眼科中心　北京市眼科学与视觉科学重点实验室
    100191　北京大学第三医院眼科
  • 收稿日期:2023-08-10 出版日期:2023-08-28
  • 通信作者: 翟长斌
  • 基金资助:
    国家自然科学基金青年项目(82201145)

Changes of serum trace elements in patients with Sjögren′s syndrome and its correlation with disease severity

Ran Hao, Shanshan Wei, Qianru Wu, Xuemin Li, Changbin Zhai†()   

  1. Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100730, China
    Department of Ophthalmology, Peking University Third Hospital, Beijing 100191, China
  • Received:2023-08-10 Published:2023-08-28
  • Corresponding author: Changbin Zhai
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引用本文：

郝然, 魏姗珊, 吴倩如, 李学民, 翟长斌. 干燥综合征血清微量元素变化及其与疾病严重程度的相关性研究[J/OL]. 中华眼科医学杂志(电子版), 2023, 13(04): 215-220.

Ran Hao, Shanshan Wei, Qianru Wu, Xuemin Li, Changbin Zhai. Changes of serum trace elements in patients with Sjögren′s syndrome and its correlation with disease severity[J/OL]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2023, 13(04): 215-220.

目的

探讨干燥综合征(SS)患者血清微量元素水平变化及其与疾病严重程度的关系。

方法

收集2022年10月至2023年8月于首都医科大学附属北京同仁医院眼科中心就诊的干眼患者65例(65只眼)。其中，男性5例(5只眼)，女性60例(60只眼)，年龄39~75岁，平均(54.3±18.4)岁。根据美国风湿病学会和欧洲风湿病联盟(ACR－EULAR)分类标准分为SS组和对照组。全部患者均进行眼表疾病指数(OSDI)评分和ACR－EULAR评分，检测下睑中央泪河高度(TMH)和泪膜破裂时间(TBUT)，化学试验检测铜、锌、铁、钙及镁等血清微量元素。年龄、OSDI评分、TMH、TBUT、泪液分泌试验(ST)、眼表染色评分(OSS)及血清微量元素浓度等均服从正态分布，采用±s进行描述，组间比较采用独立样本t检验。性别采用例数描述，组间比较采用卡方检验。血清微量元素浓度与OSDI评分、TMH、TBUT、ST及OSS之间的相关性采用Pearson分析，与抗干燥综合征A抗体、抗干燥综合征B抗体、类风湿因子(RF)、抗核抗体(ANA)、唇腺活检及唾液腺流率之间的相关性采用Spearman分析。

结果

SS组和对照组患者血清铜水平分别为(1.67±0.32)μg/ml和(1.12±0.51)μg/ml，SS组血清铜浓度高于对照组，差异有统计学意义(t＝2.817，P<0.05)。SS组和对照组患者血清锌水平分别为(0.88±0.15)μg/ml和(0.99±0.13)μg/ml，SS组血清锌浓度低于对照组，差异有统计学意义(t＝－2.245，P<0.05)。SS组和对照组患者血清铁水平分别为(0.87±0.12)μg/ml和(1.04±0.24)μg/ml，SS组血清铁浓度低于对照组，差异有统计学意义(t＝－2.087，P<0.05)。SS组和对照组患者血清钙水平分别为(77.22±12.15)μg/ml和(92.07±15.42)μg/ml，SS组血清钙浓度低于对照组，差异有统计学意义(t＝－2.312，P<0.05)。SS组和对照组患者血清镁水平分别为(17.33±2.12)μg/ml和(20.51±3.14)μg/ml，SS组血清镁浓度低于对照组，差异有统计学意义(t＝－3.507，P<0.05)。血清铜与唇腺活检及OSDI呈正相关，其相关性具有统计学意义(r＝0.325，0.389；P<0.05)；与ST、TMH及TBUT呈负相关，其相关性具有统计学意义(r＝－0.471，－0.340，－0.408；P<0.05)。血清钙与OSS及TBUT呈负相关，其相关性具有统计学意义(r＝－0.389，－0.428；P<0.05)。血清镁与ST、唾液腺流率、TMH及TBUT呈正相关，其相关性具有统计学意义(r＝0.316，0.405，0.322，0.479；P<0.05)；与抗干燥综合征A抗体、RF、唇腺活检、OSS及OSDI呈负相关，其相关性具有统计学意义(r＝－0.351，－0.324，－0.429，－0.298，－0.422；P<0.05)。血清铁与唇腺活检及OSS呈负相关，其相关性具有统计学意义(r＝－0.320，－0.356；P<0.05)。

结论

血清微量元素水平与SS疾病的严重程度相关，监测微量元素的变化有助于了解疾病的严重程度并指导治疗。

关键词: 干燥综合征, 微量元素, 免疫, 炎症
Objective

To investigate the levels of serum trace element in patients with Sjögren′s Syndrome (SS) and its correlations with the severity of SS.

Methods

Sixty-five dry eye patients (65 eyes) registered the Eye Center of Beijing Tongren Hospital affiliated to Capital Medical University from October 2022 to August 2023 were selected. Among them, there were 5 males (5 eyes) and 60 females (60 eyes), aged 39 to 75 years, with an average age of (54.3±18.4) years. According to the classification standards of the American Rheumatology Society and the European Rheumatology Alliance (ACR-EULAR), they were divided into SS group and the control group. All patients were scored with the eye surface disease index (OSDI) and ACR-EULAR scores, and the central tear river height (TMH) and tear film rupture time (TBUT) of the lower eyelid were measured. The serum trace elements such as copper, zinc, iron, calcium, and magnesium were detected by chemical tests. Age, OSDI score, TMH, TBUT, tear secretion test (ST), eye surface staining score (OSS), and serum trace element concentration conforming to normal distribution were described by ±s, and compared by independent sample t test for groups. Gender was described using case numbers, and compared by chi square tests. The correlation between serum trace element concentrations and OSDI scores, TMH, TBUT, ST, and OSS was analyzed using Pearson analysis. The correlation between serum trace element concentrations and anti Sjogren′s syndrome A antibodies, anti Sjogren′s syndrome B antibodies, rheumatoid factor (RF), antinuclear antibodies (ANA), labial gland biopsy, and salivary gland flow rate was analyzed using Spearman analysis.

Results

The serum copper levels in SS group and control group were (1.67±0.32)μg/ml and (1.12±0.51) μg/ml, respectively; the former concentration was higher than the latter. There was a statistically significant difference in the serum copper levels between groups (t=2.817, P<0.05). The serum zinc levels of patients in SS group and control group were (0.88±0.15)μg/ml and (0.99±0.13)μg/ml, respectively; the former concentration was lower than the latter. There was a statistically significant difference in the serum copper levels between groups (t=-2.245, P<0.05). The serum iron levels of patients in SS group and control group were (0.87±0.12)μg/ml and (1.04±0.24) μg/ml, respectively; the former concentration was lower than the latter. There was a statistically significant difference in the serum iron levels between groups (t=-2.087, P<0.05). The serum calcium levels of patients in SS and control group were (77.22±12.15)μg/ml and (92.07±15.42) μg/ml, respectively; the former concentration was lower than the latter. There was a statistically significant difference in the serum calcium levels between groups (t=-2.312, P<0.05). The serum magnesium levels in SS group and control group were (17.33±2.12)μg/ml and (20.51±3.14) μg/ml, respectively; the former concentration was lower than the latter. There was a statistically significant difference in the serum magnesium levels between groups (t=-3.507, P<0.05). Serum copper was positively correlated with labial gland biopsy and OSDI, and the correlation was statistically significant (r=0.325, 0.389; P<0.05). There was a negative correlation with ST, TMH, and TBUT, and the correlation was statistically significant (r=-0.471, -0.340, -0.408; P<0.05). Serum calcium was negatively correlated with OSS and TBUT, and the correlation was statistically significant (r=-0.389, -0.428; P<0.05). Serum magnesium was positively correlated with ST, salivary gland flow rate, TMH, and TBUT, and the correlation was statistically significant (r=0.316, 0.405, 0.322, 0.479; P<0.05). There was a negative correlation with anti Sjogren′s syndrome A antibodies, RF, labial gland biopsy, OSS, and OSDI, and the correlation was statistically significant (r=-0.351, -0.324, -0.429, -0.298, -0.422; P<0.05). Serum iron was negatively correlated with lip gland biopsy and OSS, and the correlation was statistically significant (r=-0.320, -0.356; P<0.05).

Conclusions

The levels of trace element were related to the severity of SS, monitoring changes in trace elements can help understand the severity of the disease and guide treatment.

Key words: Sj?gren′s syndrome, Trace elements, Immunity, Inflammation
表1 SS组和对照组患者常用检查项目检查结果的比较( ±s)
组别 例数 OSDI(分) TMH(mm) TBUT(s) OSS(分) ST(mm)
SS组 31 67.27±27.30 0.08±0.04 2.52±1.56 6.88±2.70 1.87±0.76
对照组 34 38.16±16.47 0.15±0.05 4.46±2.44 2.26±0.84 3.44±1.16
t值   5.187 －2.911 －6.545 8.566 －3.586
P值   <0.05 <0.05 <0.05 <0.05 <0.05
表2 SS组和对照组患者血清微量元素检查结果的比较(±s，μg/ml)
组别 例数 铜 锌 铁 钙 镁
SS组 31 1.67±0.32 0.88±0.15 0.87±0.12 77.22±12.15 17.33±2.12
对照组 34 1.12±0.51 0.99±0.13 1.04±0.24 92.07±15.42 20.51±3.14
t值   2.817 －2.245 －2.087 －2.312 －3.507
P值   <0.05 <0.05 <0.05 <0.05 <0.05
表3 血清微量元素与干燥综合征常用检测指标相关分析的结果
干燥综合征常用检测指标 统计值 铜 锌 钙 镁 铁
抗干燥综合征A抗体 r值 0.264 0.053 0.053 －0.351 0.249
P值 >0.05 >0.05 >0.05 <0.05 >0.05
抗干燥综合征B抗体 r值 0.231 －0.110 0.065 0.268 0.147
P值 >0.05 >0.05 >0.05 >0.05 >0.05
类风湿因子 r值 0.252 －0.012 0.053 －0.324 0.243
P值 >0.05 >0.05 >0.05 <0.05 >0.05
抗核抗体 r值 0.090 0.095 －0.047 0.275 0.233
P值 >0.05 >0.05 >0.05 >0.05 >0.05
唇腺活检 r值 0.325 0.130 0.032 －0.429 －0.320
P值 <0.05 >0.05 >0.05 <0.05 <0.05
泪液分泌试验 r值 －0.471 0.011 －0.184 0.316 0.171
P值 <0.05 >0.05 >0.05 <0.05 >0.05
眼表染色评分 r值 0.163 －0.004 －0.389 －0.298 －0.356
P值 >0.05 >0.05 <0.05 <0.05 <0.05
唾液腺流率 r值 －0.155 －0.170 －0.040 0.405 0.308
P值 >0.05 >0.05 >0.05 <0.05 >0.05
眼表疾病指数 r值 0.389 －0.143 0.200 －0.422 －0.276
P值 <0.05 >0.05 >0.05 <0.05 >0.05
下睑中央泪河高度 r值 －0.340 －0.104 －0.189 0.332 0.275
P值 <0.05 >0.05 >0.05 <0.05 >0.05
泪膜破裂时间 r值 －0.408 0.009 －0.428 0.479 0.247
P值 <0.05 >0.05 <0.05 <0.05 >0.05
[1]
Shiboski CH, Shiboski SC, Seror R, et al. 2016 American college of rheumatology/European league against rheumatism classification criteria for primary Sjögren′s syndrome: a consensus and data－driven methodology involving three international patient cohorts[J]. Arthritis Rheumatol, 2017, 69(1): 35－45.
[2]
Soret P, Le DC, Desvaux E, et al. A new molecular classification to drive precision treatment strategies in primary Sjögren′s syndrome[J]. Nat Commun, 2021, 12(1): 1－18.
[3]
Hao R, Liu Z, Chou Y, et al. Impression cytology and in vivo confocal microscopy of lip mucosa compared with labial gland biopsy and classification criteria in patients with clinically suspected primary Sjögren′s syndrome[J]. Front Immunol, 2022, 13(2): 1－14.
[4]
Pedro EM, da Rosa Franchi Santos LF, Scavuzzi BM, et al. Trace elements associated with systemic lupus erythematosus and insulin resistance[J]. Biol Trace Elem Res, 2019, 191(1): 34－44.
[5]
Noémi Tóth CN, Baranyai E, Csípö I, et al. Elemental analysis of whole and protein separated blood serum of patients with systemic lupus erythematosus and Sj？gren′s syndrome[J]. Biol Trace Elem Res, 2017, 179(1): 14－22.
[6]
Yilmaz A, Sari RA, Gundogdu M, et al. Trace elements and some extracellular antioxidant proteins levels in serum of patients with systemic lupus erythematosus[J]. Clin Rheumatol, 2005, 24(4): 331－335.
[7]
Wang H, Li XB, Huang RG, et al. Essential trace element status in systemic lupus erythematosus: a Meta－analysis based on case－control studies[J]. Biol Trace Elem Res, 2023, 201(5): 2170－2182.
[8]
Wolffsohn JS, Arita R, Chalmers R, et al. TFOS DEWS II Diagnostic Methodology report[J]. Ocul Surf, 2017, 15(3): 539－574.
[9]
Bron AJ, de Paiva CS, Chauhan SK, et al. TFOS DEWS Ⅱ pathophysiology report[J]. Ocul Surf, 2017, 15(3): 438－510.
[10]
Willcox MD, Argüeso P, Georgiev GA, et al. TFOS DEWS Ⅱ Tear Film Report[J]. Ocul Surf, 2017, 15(3): 366－403.
[11]
Hao R, Ding Y, Li X. Alterations in corneal epithelial dendritic cell in Sjogren′s syndrome dry eye and clinical correlations[J]. Sci Rep, 2022, 12(1): 1－10.
[12]
Hao R, Liu Z, Chou Y, et al. Analysis of globular cells in corneal nerve vortex[J]. Front Med, 2022, 9(1): 1－11.
[13]
Vural H, Uzun K, Uz E, et al. Concentrations of copper, zinc and various elements in serum of patients with bronchial asthma[J]. J Trace Elem Med Biol, 2000, 4(2): 88－91.
[14]
Kurien BT, Scofield RH. Autoimmunity and oxidatively modified autoantigens[J]. Autoimmun Rev, 2008, 7(7): 567－573.
[15]
Pereira TC, Campos MM, Bogo MR. Copper toxicology, oxidative stress and inflammation using zebrafish as experimental model[J]. J Appl Toxicol, 2016, 36(7): 876－885.
[16]
Wei H, Zhang WJ, Leboeuf R, et al. Copper induces－－and copper chelation by tetrathiomolybdate inhibits－endothelial activation in vitro[J]. Redox Rep, 2014, 19(1): 40－48.
[17]
Bafaro E, Liu Y, Xu Y, et al. The emerging role of zinc transporters in cellular homeostasis and cancer[J]. Signal Transduct Target Ther, 2017, 2(2): 1－12.
[18]
Feinauer CJ, Hofmann A, Goldt S, et al. Zinc finger proteins and the 3D organization of chromosomes[J]. Adv Protein Chem Struct Biol, 2013, 90: 67－117.
[19]
Wessels I, Maywald M, Rink L. Zinc as a gatekeeper of immune function[J]. Nutrients, 2017, 9(12): 1－44.
[20]
Gammoh NZ, Rink L. Zinc in infection and inflammation[J]. Nutrients, 2017, 9(6): 1－25.
[21]
Cornelissen A, Guo L, Sakamoto A, et al. New insights into the role of iron in inflammation and atherosclerosis[J]. EBioMedicine, 2019, 47(9): 598－606.
[22]
Haschka D, Hoffmann A, Weiss G. Iron in immune cell function and host defense[J]. Semin Cell Dev Biol, 2021, 115(2): 27－36.
[23]
Fayyaz A, Igoe A, Kurien BT, et al. Haematological manifestations of lupus[J]. Lupus Sci Med, 2015, 2(1): 1－18.
[24]
Voulgarelis M, Kokori SI, Ioannidis JP, et al. Anaemia in systemic lupus erythematosus: aetiological profile and the role of erythropoietin[J]. Ann Rheum Dis, 2000, 59(3): 217－222.
[25]
Velo－García A, Castro SG, Isenberg DA. The diagnosis and management of the haematologic manifestations of lupus[J]. J Autoimmun, 2016, 74(2): 139－160.
[26]
Moe SM. Disorders involving calcium, phosphorus, and magnesium. Prim Care[J], 2008, 35(2): 215－237.
[27]
Ambudkar I. Calcium signaling defects underlying salivary gland dysfunction[J]. Biochim Biophys Acta Mol Cell Res, 2018, 1865(1): 1771－1777.
[28]
Gallo A, Jang SI, Ong HL, et al. Targeting the Ca2+ sensor STIM1 by exosomal transfer of Ebv－miR－BART13－3p is associated with Sjögren′s syndrome[J]. EBioMedicine, 2016, 10(2): 216－226.
[29]
Fritzen R, Davies A, Veenhuizen M, et al. Magnesium deficiency and cardiometabolic disease[J]. Nutrients, 2023, 15(10): 1－19.
[30]
Ryan MF. The role of magnesium in clinical biochemistry: an overview[J]. Ann Clin Biochem. 1991, 28(1): 19－26.
[31]
Romano TJ. Magnesium Deficiency in Systemic Lupus Erythematosus[J]. J Nutr Environ Med. 1997, 7(1): 107－111.
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[15] 谭瑞义. 小细胞骨肉瘤诊断及治疗研究现状与进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(08): 781-784.
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