切换至 "中华医学电子期刊资源库"

中华眼科医学杂志(电子版) ›› 2022, Vol. 12 ›› Issue (06) : 372 -376. doi: 10.3877/cma.j.issn.2095-2007.2022.06.010

综述

眼表菌群改变与干眼关系的研究进展
荆大兰1, 江晓丹2, 杨嘉瑞2, 李学民2,()   
  1. 1. 100191 北京大学第三医院眼科中心2020级博士研究生
    2. 100191 北京大学第三医院眼科中心 眼部神经损伤的重建与康复北京市重点实验室
  • 收稿日期:2021-08-04 出版日期:2022-12-28
  • 通信作者: 李学民
  • 基金资助:
    北京市自然科学基金项目(7202229)

Research progress on the relationship between changes of ocular surface flora and dry eye

Dalan Jing1, Xiaodan Jiang2, Jiarui Yang2, Xuemin Li2,()   

  1. 1. Doctoral degree 2020, Peking University Third Hospital, Peking University Eye Center, Beijing 100191, China
    2. Peking University Third Hospital, Peking University Eye Center, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Beijing 100191, China
  • Received:2021-08-04 Published:2022-12-28
  • Corresponding author: Xuemin Li
引用本文:

荆大兰, 江晓丹, 杨嘉瑞, 李学民. 眼表菌群改变与干眼关系的研究进展[J]. 中华眼科医学杂志(电子版), 2022, 12(06): 372-376.

Dalan Jing, Xiaodan Jiang, Jiarui Yang, Xuemin Li. Research progress on the relationship between changes of ocular surface flora and dry eye[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2022, 12(06): 372-376.

干眼是一种多因素的眼表疾病,近年来发病率有急剧上升趋势,已成为日益严重的公共卫生问题。有研究结果表明,干眼患者眼表菌群的状态与其干眼症状的严重程度相关。因此,从眼表菌群改变角度探究干眼发病机制成为研究的新热点。本文中笔者以干眼患者眼表分离到的菌种为切入点,阐述眼表菌群改变与干眼的关系,旨在为管理干眼危险因素和开拓临床治疗方法提供参考。

Dry eye is a multifactorial ocular surface disease. Currently, its incidence rate is rising rapidly, and has become an increasingly serious public health problem. The relevant literatures has shown that the state of dry eye patients′ ocular surface flora is related to the severity of the disease. Therefore, to investigate the pathogenesis of dry eye, from the perspective of ocular surface flora has been a hot research topic. In this paper, the relationship between changes of ocular surface flora and dry eye sign from the perspective of bacterium isolated from ocular surface of patients with dry eye was reviewed, in order to provide a basis for the management of dry eye risk factors and clinical treatment.

图1 眼表菌群改变与干眼关系及机制示意图
[1]
Zegans ME, van-Gelder RN. Considerations in understanding the ocular surface microbiome[J]. Am J Ophthalmol, 2014, 158: 420-422.
[2]
安娜. 眼表菌群的研究进展[J]. 中华实验眼科杂志201836(9):714-718.
[3]
Mshangila B, Paddy M, Kajumbula H, et al. External ocular surface bacterial isolates and their antimicrobial susceptibility patterns among pre-operative cataract patients at Mulago National Hospital in Kampala, Uganda[J]. BMC Ophthalmol, 201313: 71.
[4]
刘祖国,王华. 关注干眼慢性疾病管理体系的建设[J] 中华眼科杂志201854(2):81-83.
[5]
McDermott AM. Antimicrobial compounds in tears[J]. Exp Eye Research, 2013, 117: 53-61.
[6]
Dong Q, Brulc JM, Iovieno A, et al. Diversity of bacteria at healthy human conjunctiva[J]. Invest OphthalmolVis Sci, 2011, 52(8): 5408-5413.
[7]
Doan T, Akileswaran L, Andersen D, et al. Paucibacterial microbiome and resident DNA virome of the healthy conjunctiva[J]. Invest Ophthalmol Vis Sci, 2016, 57(13): 5116-5126.
[8]
Kaufman HE, Azcuy AM, Varnell ED, et al. HSV-1 DNA in tears and saliva of normal adults[J].Invest Ophthalmol Vis Sci, 2005, 46(1): 241-247.
[9]
Moss JM, Sanislo SR, Ta CN. Antibiotic susceptibility patterns of ocular bacterial flora in patients undergoing intravitreal injections[J]. Ophthalmology, 2010, 117(11): 2141-2145.
[10]
张建华,郑磊,高鹏,等. LASIK术前眼表细菌培养及围手术期用药分析[J]. 眼科新进展200828(6):438-440.
[11]
Suto C, Morinaga M, Yagi T, et al. Conjunctival sac bacterial flora isolated prior to cataract surgery[J]. Infect Drug Resist, 2012, 5: 37-41.
[12]
Venugopal R, Satpathy G, Sangwan S, et al. Conjunctival microbial flora in ocular Stevens-Johnson syndrome sequelae patients at a tertiary eye care center[J]. Cornea, 2016, 35(8): 1117-1121.
[13]
Nentwich MM, Rajab M, Ta CN, et al. Application of 10% povidone iodine reduces conjunctival bacterial contamination rate in patients undergoing cataract surgery[J]. Eur JOphthalmol, 2012, 22(4): 541-546.
[14]
Bürgmann H, Pesaro M, Widmer F, et al. A strategy for optimizing quality and quantity of DNA extracted from soil[J]. J Microbiol Methods, 2001, 45(1): 7-20.
[15]
Walker CB, Claoué CM. Incidence of conjunctival colonization by bacteria capable of causing postoperative endophthalmitis[J]. J R Soc Med, 1986, 79(9): 520-521.
[16]
Miller D, Iovieno A. The role of microbial flora on the ocular surface[J]. Curr Opin Allergy Clin Immunol, 2009, 9(5): 466-470.
[17]
Berry M, Harris A, Lumb R, et al. Commensal ocular bacteria degrade mucins[J]. Br J Ophthalmol, 2002, 86(12): 1412-1416.
[18]
厉芸芸. 急性结膜炎后干眼症状探讨[J]. 医学理论与实践201225(11):1340-1341.
[19]
O′Callaghan RJ, Girgis DO, Dajcs JJ, et al. Host defense against bacterial keratitis[J]. Ocul Immunol Inflamm, 2003, 11(3): 171-181.
[20]
Groden LR, Murphy B, Rodnite J, et al. Lid flora in blepharitis[J]. Cornea, 1991, 10(1): 50-53.
[21]
Ta CN, Shine WE, McCulley JP, et al. Effects of minocycline on the ocular flora of patients with acne rosacea or seborrheic blepharitis[J].Cornea, 2003, 22(6): 545-548.
[22]
Jiang X, Deng A, Yang J, et al. Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction[J]. Infect Drug Resist, 2018, 11: 1729-1740.
[23]
Wolffsohn JS, Arita R, Chalmers R, et al. TFOS DEWS Ⅱ diagnostic methodology report[J]. Ocul Surf, 201715(3): 539-574.
[24]
Koh S. Mechanisms of visual disturbance in dry eye[J]. Cornea, 2016, 35(1): S83-S88.
[25]
McCulley JP, Shine WE. Eyelid disorders: the meibomian gland, blepharitis, and contact lenses[J]. Eye Contact Lens, 2003, 29(1): S93-S95.
[26]
Speaker MG, Milch FA, Shah MK, et al. Role of external bacterial flora in the pathogenesis of acute postoperative endophthalmitis[J]. Ophthalmol, 1991, 98(5): 639-649.
[27]
Abe T, Nakajima A, Matsunaga M, et al. Decreased tear lactoferrin concentration in patients with chronic hepatitis C[J]. Br J Ophthalmol, 1999, 83(6): 684-687.
[28]
Holden BA, Sweeney DF, Sankaridurg PR, et al. Microbial keratitis and vision loss with contact lenses[J]. Eye Contact Lens, 200329(1): S131-S134.
[29]
Knox CM, Cevellos V, Dean D. 16S ribosomal DNA typing for identification of pathogens in patients with bacterial keratitis[J]. J Clin Microbiol, 1998, 36(12): 3492-3496.
[30]
Aristoteli LP, Bojarski B, Willcox MD. Isolation of conjunctival mucin and differential interaction with Pseudomonas aeruginosa strains of varied pathogenic potential[J]. Exp Eye Res, 2003, 77(6): 699-710.
[31]
Dougherty JM, McCulley JP, Silvany RE, et al. The role of tetracycline in chronic blepharitis. Inhibition of lipase production in staphylococci[J]. Invest Ophthalmol Vis Sci, 1991, 32(11): 2970-2975.
[32]
Chen Y, Chauhan SK, Lee HS, et al. Chronic dry eye disease is principally mediated by effector memory Th17 cells[J]. Mucosal Immunol, 2014, 7(1): 38-45.
[33]
Cheng H, Guan X, Chen D, et al. The Th17/Treg cell balance: a gut microbiota-modulated story[J]. Microorganisms, 2019, 7(12): 583.
[34]
Ueta M. Innate immunity of the ocular surface and ocular surface inflammatory disorders[J]. Cornea, 2008, 27(1): S31-S40.
[35]
胡燕华,贺乐荷. 108例干眼症之结膜囊细菌菌落[J]. 眼科研究199513(3):204-206.
[36]
Zhang SD, He JN, Niu TT, et al. Bacteriological profile of ocular surface flora in meibomian gland dysfunction[J]. Ocul Surf, 2017, 15(2): 242-247.
[37]
Dougherty JM, McCulley JP. Comparative bacteriology of chronic blepharitis[J]. Br J Ophthalmol, 1984, 68(8): 524-528.
[38]
董万江,张悦,刘治容,等. 绵阳市汉族中老年人干眼症与非干眼症结膜囊细菌状况对照研究 [J]. 眼科新进展201131(10):965-968.
[39]
Graham JE, Moore JE, Jiru X, et al. Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes[J]. Invest Ophthalmol Vis Sci, 2007, 48(12): 5616-5623.
[40]
Reading NC, Sperandio V. Quorum sensing: the many languages of bacteria[J]. FEMS Microbiol Lett, 2006, 254(1): 1-11.
[41]
Albietz JM, Lenton LM. Effect of antibacterial honey on the ocular flora in tear deficiency and meibomian gland disease[J]. Cornea, 2006, 25(9): 1012-1019.
[42]
Diard M, Hardt WD. Evolution of bacterial virulence[J]. FEMS Microbiol Rev, 2017, 41(5): 679-697.
[43]
Cuello OH, Caorlin MJ, Reviglio VE, et al. Rhodococcus globerulus keratitis after laser in situ keratomileusis[J]. J Cataract Refract Surg, 2002, 28(12): 2235-2237.
[44]
Willis KA, Postnikoff CK, Freeman A, et al. The closed eye harbours a unique microbiome in dry eye disease[J]. Sci Rep, 2020, 10(1): 12035.
[45]
Costello EK, Stagaman K, Dethlefsen L, et al. The application of ecological theory toward an understanding of the human microbiome[J]. Science, 2012, 336(6086): 1255-1262.
[46]
Feher J, Pinter E, Kovacs I, et al. Irritable eyesyndrome: Neuroimmune mechanisms and benefits of selected nutrients[J]. Ocul Surf, 2014, 12(2): 134-145.
[47]
Redfern RL, Patel N, Hanlon SI, et al. Toll-like receptor expression andactivation in mice with experimental dry eye[J]. Invest Ophthalmol Vis Sci, 2013, 54(2): 1554-1563.
[48]
Reins RY, Lema C, Courson J, et al. MyD88 deficiency protects against dry eye-induced damage[J].Invest Ophthalmol Vis Sci, 2018, 59(7): 2967-2976.
[49]
Kawakami A, Nakashima K, Tamai M, et al. Toll-like receptor in salivaryglands from patients with Sjögren′s syndrome: Functional analysis by human salivary gland cell line[J]. J Rheumatol, 2007, 34(5): 1019-1026.
[50]
Bron AJ, de-Paiva CS, Chauhan SK, et al. TFOS DEWS II pathophysiology report[J]. Ocul Surf, 2017, 15(3): 438-510.
[51]
Ye Z, Zhang N, Wu C, et al. A metagenomic study of the gut microbiome in Behcet′s disease[J]. Microbiome, 2018, 6(1): 135.
[52]
马漠,刘煜. 肠道菌群与肥胖症的关系及其在肥胖症治疗中的应用[J]. 中国糖尿病杂志20179(11):726-728.
[53]
Moon J, Yoon CH, Choi SH, et al. Can gut microbiota affect dry eye syndrome?[J]. Int J Mol Sci, 2020, 21(22) : 8443.
[54]
Li JJ, Yi S, Wei L. Ocular microbiota and intraocular inflammation[J]. Front Immunol, 2020, 11: 609765.
[55]
Wan KH, Chen LJ, Young AL. Depression and anxiety in dry eye disease: a systematic review and meta-analysis[J]. Eye, 2016, 30(12): 1558-1567.
[56]
Miljanoviĉ B, Trivedi KA, Dana MR, et al. Relation between dietary n-3 and n-6 fatty acids and clinically diagnosed dry eye syndrome in women[J]. Am J Clin Nutr, 2005, 82(4): 887-893.
[1] 刘欢颜, 华扬, 贾凌云, 赵新宇, 刘蓓蓓. 颈内动脉闭塞病变管腔结构和血流动力学特征分析[J]. 中华医学超声杂志(电子版), 2023, 20(08): 809-815.
[2] 马艳波, 华扬, 刘桂梅, 孟秀峰, 崔立平. 中青年人颈动脉粥样硬化病变的相关危险因素分析[J]. 中华医学超声杂志(电子版), 2023, 20(08): 822-826.
[3] 唐旭, 韩冰, 刘威, 陈茹星. 结直肠癌根治术后隐匿性肝转移危险因素分析及预测模型构建[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 16-20.
[4] 吴方园, 孙霞, 林昌锋, 张震生. HBV相关肝硬化合并急性上消化道出血的危险因素分析[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 45-47.
[5] 陈旭渊, 罗仕云, 李文忠, 李毅. 腺源性肛瘘经手术治疗后创面愈合困难的危险因素分析[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 82-85.
[6] 叶晓琳, 刘云飞, 庞明泉, 王海久, 任利, 侯立朝, 于文昊, 王志鑫, 樊海宁. 肝再生细胞来源及调控机制的研究进展[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 96-99.
[7] 彭旭, 邵永孚, 李铎, 邹瑞, 邢贞明. 结肠肝曲癌的诊断和外科治疗[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 108-110.
[8] 马伟强, 马斌林, 吴中语, 张莹. microRNA在三阴性乳腺癌进展中发挥的作用[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 111-114.
[9] 陆猛桂, 黄斌, 李秋林, 何媛梅. 蜂蛰伤患者发生多器官功能障碍综合征的危险因素分析[J]. 中华临床医师杂志(电子版), 2023, 17(9): 1010-1015.
[10] 李达, 张大涯, 陈润祥, 张晓冬, 黄士美, 陈晨, 曾凡, 陈世锔, 白飞虎. 海南省东方市幽门螺杆菌感染现状的调查与相关危险因素分析[J]. 中华临床医师杂志(电子版), 2023, 17(08): 858-864.
[11] 陆志峰, 周佳佳, 梁舒. 虚拟现实技术在治疗弱视中的临床应用研究进展[J]. 中华临床医师杂志(电子版), 2023, 17(08): 891-895.
[12] 李田, 徐洪, 刘和亮. 尘肺病的相关研究进展[J]. 中华临床医师杂志(电子版), 2023, 17(08): 900-905.
[13] 岳瑞雪, 孔令欣, 郝鑫, 杨进强, 韩猛, 崔国忠, 王建军, 张志生, 孔凡庭, 张维, 何文博, 李现桥, 周新平, 徐东宏, 胡崇珠. 乳腺癌HER2蛋白表达水平预测新辅助治疗疗效的真实世界研究[J]. 中华临床医师杂志(电子版), 2023, 17(07): 765-770.
[14] 李琪, 黄钟莹, 袁平, 关振鹏. 基于某三级医院的ICU多重耐药菌医院感染影响因素的分析[J]. 中华临床医师杂志(电子版), 2023, 17(07): 777-782.
[15] 符梅沙, 周玉华, 李慧, 薛春颜. 淋巴细胞免疫治疗对复发性流产患者外周血T淋巴细胞亚群分布与PD1/PD-L1表达的影响及意义[J]. 中华临床医师杂志(电子版), 2023, 17(06): 726-730.
阅读次数
全文


摘要