切换至 "中华医学电子期刊资源库"

中华眼科医学杂志(电子版) ›› 2022, Vol. 12 ›› Issue (06) : 372 -376. doi: 10.3877/cma.j.issn.2095-2007.2022.06.010

综述

眼表菌群改变与干眼关系的研究进展
荆大兰1, 江晓丹2, 杨嘉瑞2, 李学民2,()   
  1. 1. 100191 北京大学第三医院眼科中心2020级博士研究生
    2. 100191 北京大学第三医院眼科中心 眼部神经损伤的重建与康复北京市重点实验室
  • 收稿日期:2021-08-04 出版日期:2022-12-28
  • 通信作者: 李学民
  • 基金资助:
    北京市自然科学基金项目(7202229)

Research progress on the relationship between changes of ocular surface flora and dry eye

Dalan Jing1, Xiaodan Jiang2, Jiarui Yang2, Xuemin Li2,()   

  1. 1. Doctoral degree 2020, Peking University Third Hospital, Peking University Eye Center, Beijing 100191, China
    2. Peking University Third Hospital, Peking University Eye Center, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Beijing 100191, China
  • Received:2021-08-04 Published:2022-12-28
  • Corresponding author: Xuemin Li
引用本文:

荆大兰, 江晓丹, 杨嘉瑞, 李学民. 眼表菌群改变与干眼关系的研究进展[J/OL]. 中华眼科医学杂志(电子版), 2022, 12(06): 372-376.

Dalan Jing, Xiaodan Jiang, Jiarui Yang, Xuemin Li. Research progress on the relationship between changes of ocular surface flora and dry eye[J/OL]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2022, 12(06): 372-376.

干眼是一种多因素的眼表疾病,近年来发病率有急剧上升趋势,已成为日益严重的公共卫生问题。有研究结果表明,干眼患者眼表菌群的状态与其干眼症状的严重程度相关。因此,从眼表菌群改变角度探究干眼发病机制成为研究的新热点。本文中笔者以干眼患者眼表分离到的菌种为切入点,阐述眼表菌群改变与干眼的关系,旨在为管理干眼危险因素和开拓临床治疗方法提供参考。

Dry eye is a multifactorial ocular surface disease. Currently, its incidence rate is rising rapidly, and has become an increasingly serious public health problem. The relevant literatures has shown that the state of dry eye patients′ ocular surface flora is related to the severity of the disease. Therefore, to investigate the pathogenesis of dry eye, from the perspective of ocular surface flora has been a hot research topic. In this paper, the relationship between changes of ocular surface flora and dry eye sign from the perspective of bacterium isolated from ocular surface of patients with dry eye was reviewed, in order to provide a basis for the management of dry eye risk factors and clinical treatment.

图1 眼表菌群改变与干眼关系及机制示意图
[1]
Zegans ME, van-Gelder RN. Considerations in understanding the ocular surface microbiome[J]. Am J Ophthalmol, 2014, 158: 420-422.
[2]
安娜. 眼表菌群的研究进展[J]. 中华实验眼科杂志201836(9):714-718.
[3]
Mshangila B, Paddy M, Kajumbula H, et al. External ocular surface bacterial isolates and their antimicrobial susceptibility patterns among pre-operative cataract patients at Mulago National Hospital in Kampala, Uganda[J]. BMC Ophthalmol, 201313: 71.
[4]
刘祖国,王华. 关注干眼慢性疾病管理体系的建设[J] 中华眼科杂志201854(2):81-83.
[5]
McDermott AM. Antimicrobial compounds in tears[J]. Exp Eye Research, 2013, 117: 53-61.
[6]
Dong Q, Brulc JM, Iovieno A, et al. Diversity of bacteria at healthy human conjunctiva[J]. Invest OphthalmolVis Sci, 2011, 52(8): 5408-5413.
[7]
Doan T, Akileswaran L, Andersen D, et al. Paucibacterial microbiome and resident DNA virome of the healthy conjunctiva[J]. Invest Ophthalmol Vis Sci, 2016, 57(13): 5116-5126.
[8]
Kaufman HE, Azcuy AM, Varnell ED, et al. HSV-1 DNA in tears and saliva of normal adults[J].Invest Ophthalmol Vis Sci, 2005, 46(1): 241-247.
[9]
Moss JM, Sanislo SR, Ta CN. Antibiotic susceptibility patterns of ocular bacterial flora in patients undergoing intravitreal injections[J]. Ophthalmology, 2010, 117(11): 2141-2145.
[10]
张建华,郑磊,高鹏,等. LASIK术前眼表细菌培养及围手术期用药分析[J]. 眼科新进展200828(6):438-440.
[11]
Suto C, Morinaga M, Yagi T, et al. Conjunctival sac bacterial flora isolated prior to cataract surgery[J]. Infect Drug Resist, 2012, 5: 37-41.
[12]
Venugopal R, Satpathy G, Sangwan S, et al. Conjunctival microbial flora in ocular Stevens-Johnson syndrome sequelae patients at a tertiary eye care center[J]. Cornea, 2016, 35(8): 1117-1121.
[13]
Nentwich MM, Rajab M, Ta CN, et al. Application of 10% povidone iodine reduces conjunctival bacterial contamination rate in patients undergoing cataract surgery[J]. Eur JOphthalmol, 2012, 22(4): 541-546.
[14]
Bürgmann H, Pesaro M, Widmer F, et al. A strategy for optimizing quality and quantity of DNA extracted from soil[J]. J Microbiol Methods, 2001, 45(1): 7-20.
[15]
Walker CB, Claoué CM. Incidence of conjunctival colonization by bacteria capable of causing postoperative endophthalmitis[J]. J R Soc Med, 1986, 79(9): 520-521.
[16]
Miller D, Iovieno A. The role of microbial flora on the ocular surface[J]. Curr Opin Allergy Clin Immunol, 2009, 9(5): 466-470.
[17]
Berry M, Harris A, Lumb R, et al. Commensal ocular bacteria degrade mucins[J]. Br J Ophthalmol, 2002, 86(12): 1412-1416.
[18]
厉芸芸. 急性结膜炎后干眼症状探讨[J]. 医学理论与实践201225(11):1340-1341.
[19]
O′Callaghan RJ, Girgis DO, Dajcs JJ, et al. Host defense against bacterial keratitis[J]. Ocul Immunol Inflamm, 2003, 11(3): 171-181.
[20]
Groden LR, Murphy B, Rodnite J, et al. Lid flora in blepharitis[J]. Cornea, 1991, 10(1): 50-53.
[21]
Ta CN, Shine WE, McCulley JP, et al. Effects of minocycline on the ocular flora of patients with acne rosacea or seborrheic blepharitis[J].Cornea, 2003, 22(6): 545-548.
[22]
Jiang X, Deng A, Yang J, et al. Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction[J]. Infect Drug Resist, 2018, 11: 1729-1740.
[23]
Wolffsohn JS, Arita R, Chalmers R, et al. TFOS DEWS Ⅱ diagnostic methodology report[J]. Ocul Surf, 201715(3): 539-574.
[24]
Koh S. Mechanisms of visual disturbance in dry eye[J]. Cornea, 2016, 35(1): S83-S88.
[25]
McCulley JP, Shine WE. Eyelid disorders: the meibomian gland, blepharitis, and contact lenses[J]. Eye Contact Lens, 2003, 29(1): S93-S95.
[26]
Speaker MG, Milch FA, Shah MK, et al. Role of external bacterial flora in the pathogenesis of acute postoperative endophthalmitis[J]. Ophthalmol, 1991, 98(5): 639-649.
[27]
Abe T, Nakajima A, Matsunaga M, et al. Decreased tear lactoferrin concentration in patients with chronic hepatitis C[J]. Br J Ophthalmol, 1999, 83(6): 684-687.
[28]
Holden BA, Sweeney DF, Sankaridurg PR, et al. Microbial keratitis and vision loss with contact lenses[J]. Eye Contact Lens, 200329(1): S131-S134.
[29]
Knox CM, Cevellos V, Dean D. 16S ribosomal DNA typing for identification of pathogens in patients with bacterial keratitis[J]. J Clin Microbiol, 1998, 36(12): 3492-3496.
[30]
Aristoteli LP, Bojarski B, Willcox MD. Isolation of conjunctival mucin and differential interaction with Pseudomonas aeruginosa strains of varied pathogenic potential[J]. Exp Eye Res, 2003, 77(6): 699-710.
[31]
Dougherty JM, McCulley JP, Silvany RE, et al. The role of tetracycline in chronic blepharitis. Inhibition of lipase production in staphylococci[J]. Invest Ophthalmol Vis Sci, 1991, 32(11): 2970-2975.
[32]
Chen Y, Chauhan SK, Lee HS, et al. Chronic dry eye disease is principally mediated by effector memory Th17 cells[J]. Mucosal Immunol, 2014, 7(1): 38-45.
[33]
Cheng H, Guan X, Chen D, et al. The Th17/Treg cell balance: a gut microbiota-modulated story[J]. Microorganisms, 2019, 7(12): 583.
[34]
Ueta M. Innate immunity of the ocular surface and ocular surface inflammatory disorders[J]. Cornea, 2008, 27(1): S31-S40.
[35]
胡燕华,贺乐荷. 108例干眼症之结膜囊细菌菌落[J]. 眼科研究199513(3):204-206.
[36]
Zhang SD, He JN, Niu TT, et al. Bacteriological profile of ocular surface flora in meibomian gland dysfunction[J]. Ocul Surf, 2017, 15(2): 242-247.
[37]
Dougherty JM, McCulley JP. Comparative bacteriology of chronic blepharitis[J]. Br J Ophthalmol, 1984, 68(8): 524-528.
[38]
董万江,张悦,刘治容,等. 绵阳市汉族中老年人干眼症与非干眼症结膜囊细菌状况对照研究 [J]. 眼科新进展201131(10):965-968.
[39]
Graham JE, Moore JE, Jiru X, et al. Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes[J]. Invest Ophthalmol Vis Sci, 2007, 48(12): 5616-5623.
[40]
Reading NC, Sperandio V. Quorum sensing: the many languages of bacteria[J]. FEMS Microbiol Lett, 2006, 254(1): 1-11.
[41]
Albietz JM, Lenton LM. Effect of antibacterial honey on the ocular flora in tear deficiency and meibomian gland disease[J]. Cornea, 2006, 25(9): 1012-1019.
[42]
Diard M, Hardt WD. Evolution of bacterial virulence[J]. FEMS Microbiol Rev, 2017, 41(5): 679-697.
[43]
Cuello OH, Caorlin MJ, Reviglio VE, et al. Rhodococcus globerulus keratitis after laser in situ keratomileusis[J]. J Cataract Refract Surg, 2002, 28(12): 2235-2237.
[44]
Willis KA, Postnikoff CK, Freeman A, et al. The closed eye harbours a unique microbiome in dry eye disease[J]. Sci Rep, 2020, 10(1): 12035.
[45]
Costello EK, Stagaman K, Dethlefsen L, et al. The application of ecological theory toward an understanding of the human microbiome[J]. Science, 2012, 336(6086): 1255-1262.
[46]
Feher J, Pinter E, Kovacs I, et al. Irritable eyesyndrome: Neuroimmune mechanisms and benefits of selected nutrients[J]. Ocul Surf, 2014, 12(2): 134-145.
[47]
Redfern RL, Patel N, Hanlon SI, et al. Toll-like receptor expression andactivation in mice with experimental dry eye[J]. Invest Ophthalmol Vis Sci, 2013, 54(2): 1554-1563.
[48]
Reins RY, Lema C, Courson J, et al. MyD88 deficiency protects against dry eye-induced damage[J].Invest Ophthalmol Vis Sci, 2018, 59(7): 2967-2976.
[49]
Kawakami A, Nakashima K, Tamai M, et al. Toll-like receptor in salivaryglands from patients with Sjögren′s syndrome: Functional analysis by human salivary gland cell line[J]. J Rheumatol, 2007, 34(5): 1019-1026.
[50]
Bron AJ, de-Paiva CS, Chauhan SK, et al. TFOS DEWS II pathophysiology report[J]. Ocul Surf, 2017, 15(3): 438-510.
[51]
Ye Z, Zhang N, Wu C, et al. A metagenomic study of the gut microbiome in Behcet′s disease[J]. Microbiome, 2018, 6(1): 135.
[52]
马漠,刘煜. 肠道菌群与肥胖症的关系及其在肥胖症治疗中的应用[J]. 中国糖尿病杂志20179(11):726-728.
[53]
Moon J, Yoon CH, Choi SH, et al. Can gut microbiota affect dry eye syndrome?[J]. Int J Mol Sci, 2020, 21(22) : 8443.
[54]
Li JJ, Yi S, Wei L. Ocular microbiota and intraocular inflammation[J]. Front Immunol, 2020, 11: 609765.
[55]
Wan KH, Chen LJ, Young AL. Depression and anxiety in dry eye disease: a systematic review and meta-analysis[J]. Eye, 2016, 30(12): 1558-1567.
[56]
Miljanoviĉ B, Trivedi KA, Dana MR, et al. Relation between dietary n-3 and n-6 fatty acids and clinically diagnosed dry eye syndrome in women[J]. Am J Clin Nutr, 2005, 82(4): 887-893.
[1] 史学兵, 谢迎东, 谢霓, 徐超丽, 杨斌, 孙帼. 声辐射力弹性成像对不可切除肝细胞癌门静脉癌栓患者放射治疗效果的评价[J/OL]. 中华医学超声杂志(电子版), 2024, 21(08): 778-784.
[2] 李华志, 曹广, 刘殿刚, 张雅静. 不同入路下行肝切除术治疗原发性肝细胞癌的临床对比[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 52-55.
[3] 陈浩, 王萌. 胃印戒细胞癌的临床病理特征及治疗选择的研究进展[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 108-111.
[4] 刘柏隆, 周祥福. 压力性尿失禁阶梯治疗的项目介绍[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2025, 19(01): 125-125.
[5] 刘柏隆. 女性压力性尿失禁阶梯治疗之手术治疗方案选择[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2025, 19(01): 126-126.
[6] 中华医学会器官移植学分会. 肝移植术后缺血性胆道病变诊断与治疗中国实践指南[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 739-748.
[7] 陈伟杰, 何小东. 胆囊癌免疫靶向治疗进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 763-768.
[8] 王秋生. 胆道良性疾病诊疗策略[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 779-782.
[9] 刘琦, 王守凯, 王帅, 苏雨晴, 马壮, 陈海军, 司丕蕾. 乳腺癌肿瘤内微生物组的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(09): 841-845.
[10] 王誉英, 刘世伟, 王睿, 曾娅玲, 涂禧慧, 张蒲蓉. 老年乳腺癌新辅助治疗病理完全缓解的预测因素分析[J/OL]. 中华临床医师杂志(电子版), 2024, 18(07): 641-646.
[11] 颜世锐, 熊辉. 感染性心内膜炎合并急性肾损伤患者的危险因素探索及死亡风险预测[J/OL]. 中华临床医师杂志(电子版), 2024, 18(07): 618-624.
[12] 崔军威, 蔡华丽, 胡艺冰, 胡慧. 亚甲蓝联合金属定位夹及定位钩针标记在乳腺癌辅助化疗后评估腋窝转移淋巴结的临床应用价值探究[J/OL]. 中华临床医师杂志(电子版), 2024, 18(07): 625-632.
[13] 张平骥, 徐钰, 李天水, 庞文翼, 符师宁, 张梦圆. 重症患者镇静治疗现状及期望的调查研究[J/OL]. 中华临床医师杂志(电子版), 2024, 18(06): 562-567.
[14] 王昌前, 林婷婷, 宁雨露, 王颖杰, 谭文勇. 光免疫治疗在肿瘤领域的临床应用新进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(06): 575-583.
[15] 徐靖亭, 孔璐. PARP抑制剂治疗卵巢癌的耐药机制及应对策略[J/OL]. 中华临床医师杂志(电子版), 2024, 18(06): 584-588.
阅读次数
全文


摘要